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 cTnI within the sample gets labeled having an ALP-conjugated antibody and is captured onto the sensor surface over the slot thailand incubation stage.[forty nine] Clean fluid containing enzyme substrate is then applied to take away unbound substances, and concurrently, ALP certain to the antigen-antibody elaborate reacts, releasing an electrochemically detectable product. The created amperometric sign is straight proportional to the cTnI while in the sample.[fifty]

Just after incorporating streptavidin-coated microparticles, the complicated is certain to the reliable phase by way of conversation with biotin and streptavidin. The response mixture is aspirated into the measuring mobile, where the microparticles are magnetically captured on to the surface area in the electrode. Unbound substances are eradicated, scatter hitam as well as a voltage is placed on the electrode.[31]

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